Yasuike, N, Lu, H, Xia, P, Woolley, G, Methods in Enzymology (2019)
“…5.2. Characterization by UV-vis spectrophotometer 5.2.1. Equipment • UV-vis spectrophotometer (Perkin Elmer Lambda 35) • Temperature-controlled cuvette holder (from Quantum Northwest) • 10 mm quartz cuvette • 370 nm LED (from LedEngin, operating at 68 mW/cm2) 5.2.2. Materials • IAC-cross-linked protein sample in 10 mM sodium phosphate buffer, pH 7.0…”