We enjoy seeing what you do with our products. Here is some recent published work reporting use of Quantum Northwest temperature control.
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Decoupling Theranostics with Rare Earth Doped Nanoparticles
A. Skripka, V. Karabanovas, G. Jarockyte, R. Marin, V. Tam, M. Cerruti, R. Rotomskis, and R. Vetrone, Adv. Funct. Mater., 29, 1807105 (2019)
“…The temperature was changed using a temperature controlled cuvette holder (qpod 2e by Quantum Northwest, Washington, USA)…”
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Protein-Engineered Nanoscale Micelles for Dynamic 19F Magnetic Resonance and Therapeutic Drug Delivery
L. Hill, J. Frezzo, P. Katyal, D. Hoang, Z. Ben Youss Gironda, C. Xu, X. Xie, E. Delgado-Fukushima, Y. Wadghiri, and J. Montclare, ACS Nano 13, 2969-2985 (2019)
“…Proteins were characterized for their inverse transition temperature (Tt ) behavior at 320 nm using a UV−vis Cary-50 (Varian Inc.) equipped with a TC125 temperature regulator (Quantum Northwest)…”
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Oxyphor 2P: A High-Performance Probe for Deep-Tissue Longitudinal Oxygen Imaging
T. Esipova, M. Barrett, E. Erlebach, A. Masunov, B. Weber, and S. Vinogradov, Cell Metab. 29, 736-744.e7 (2019)
“…To establish independence of the phosphorescence decay time on the excitation pathway (2P vs 1P), the probe solution in a quartz optical cell (1cm path length) was placed in a temperature controlled chamber (Quantum Northwest) and excited by short ( 1ms-long) pulse trains from the laser at the wavelength matching the probe’s 2PA maximum…”
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Miscellaneous Multivariate Raman Spectroscopy Projects Including Hydrated Proton Characterization
L. Streacker, Thesis. (2018)
“…- The sample lies in a temperature controlled sample holder (LC-600, Quantum Northwest) with an adjustable temperature range of 0°C through 105°C. Back-scattered radiation from the sample passes through the 10° longpass…”
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Programming of Retention Capacity and Release Capabilities of Propargyl Acrylate Nanoparticles Decorated with Poloxamer Copolymer
O. Klep, Thesis. (2018)
“…Lifetime measurements were collected using a JobinYvon Fluorolog 3-222 Tau spectrometer. Photoluminescence dependence on temperature was measured using PTI spectrometer equipped with Quantum Northwest TC125 temperature control unit using 10 mm quartz cuvette…”
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Cohesin-dockerin interaction based method to facilitate fast domain shuffling of cellobiohydrolases
E. Kiviniemi, Thesis. (2018)
“…Thermostability of the proteins and the protein complex was studied with circular dichroism (CD). The proteins’ CD spectra at different temperatures were measured with Chirascan CD Spectrometer (Applied Photophysics, United Kingdom), using QS High Precision Cell 1 mm light path quatz SUPRASIL cuvette (100-1-40, Hellma Analytics, Germany) and TC125 temperature control heater (Quantum Northwest, USA) and AWC100 recirculating cooler (Julabo, Germany) to control the sample temperature…”
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Is a constant low-entropy process at the root of glycolytic oscillations?
H. Thoke, L. Olsen, L. Duelund, R. Stock, T. Heimburg, and L. Bagatolli, J Biol Phys. (2018)
“…Changes in cell size/shape were measured as light scattering using a 636.2 nm picosecond pulsed diode laser (Edinburgh Instruments, Edinburgh, Scotland), (1 μs between pulses) mounted on an Edinburgh FL920 Spectrofluorometer (Edinburgh Instruments, Edinburgh, Scotland) equipped with a temperature-controlled cuvette holder (Quantum Northwest). Sample volume was 2 ml and the temperature was 25 °C. For these measurements, the spectrofluorometer was operated in dark-mode, i.e., with the lamp switched off. Measurements were done in (at least) triplicates…”
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Formation of hemoglobin photoproduct is responsible for two-photon and single photon-excited fluorescence of red blood cells
E. Shirshin, B. Yakimov, S. Rodionov, N. Omelyanenko, A. Priezzhev, V. Fadeev, J. Lademann, and M. Darvin, Laser Phys. Lett. 15, 075604 (2018)
“…- Fluorescence spectra and excitation-emission matrices (EEMs) were measured using the FluoroMax-4 spectrofluorometer (Horiba Jobin-Yvon, Japan-France) with the installed Peltier-based cuvette holder (Quantum Northwest, USA)…”
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Temperature dependence of the absorbance of 185 nm photons by water and commonly occurring solutes and its influence on the VUV advanced oxidation process
A. Serrano Mora, and M. Mohseni, Environ. Sci.: Water Res. Technol. (2018)
“…- A thermostated cuvette holder (T2, Quantum Northwest) coupled with a temperature controller (TC1, Quantum Northwest) was used to maintain the solution inside the quartz cuvette (0.2, 0.5 & 1.0 cm, Starna) at the desired temperature…”
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Duplication of a Single Strand in a β-Sheet Can Produce a New Switching Function in a Photosensory Protein
A. Kumar, S. Nokhrin, R. Woloschuk, and G. Woolley, Biochemistry. (2018)
“…UV-Vis spectrophotometer (Ocean Optics Inc. USB4000), in each case coupled to a temperature controlled cuvette holder (Quantum Northwest, Inc.). Protein concentrations were determined using an extinction coefficient at λmax (~446 nm) of 45 x 10…”
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Aggregation-primed molten globule conformers of the p53 core domain provide potential tools for studying p53C aggregation in cancer
M. Pedrote, G. de Oliveira, A. Felix, M. Mota, M. Marques, I. Soares, A. Iqbal, D. Norberto, A. Gomes, E. Gratton, E. Cino, and J. Silva, J. Biol. Chem. (2018)
“…agitation. The reactions were performed in a 1 mL volume in a square cell equipped with magnetic stirrers (Hellma, #109004F-10-40), and temperature was controlled (Quantum Northwest, #TC125) at 37°C throughout the assay…”
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Complexation of Trivalent Lanthanides (Eu) and Actinides (Cm) with Aqueous Phosphates at Elevated Temperatures
N. Jordan, M. Demnitz, H. Lösch, S. Starke, V. Brendler, and N. Huittinen, Inorg Chem. 57, 7015-7024 (2018)
“…The sample temperature was kept constant at the desired temperature in a heatable cuvette holder (Quantum Northwest TL 125) during the complete duration of the measurement. The laser spectroscopic measurements were performed with a pulsed Nd:YAG (Continuum Surelite II) pump laser coupled to a solid-state optical parametric oscillator (OPO, PANTHER EX OPO, Continuum)…”
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Speciation of Reactive Sulfur Species and their Reactions with Alkylating Agents: Do we have any clue about what is present inside the cell?
V. Bogdándi, T. Ida, T. Sutton, C. Bianco, T. Ditrói, G. Koster, H. Henthorn, M. Minnion, J. Toscano, A. van der Vliet, M. Pluth, M. Feelisch, J. Fukuto, T. Akaike, and P. Nagy, Br. J. Pharmacol. (2018)
“…Inorganic polysulfide speciation in non-aqueous systems (Fig 10 and Table 2) UV-Vis measurements UV-Vis measurements were performed on a Cary 100 spectrophotometer equipped with a Quantum Northwest cuvette temperature controller under anaerobic conditions..”
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Plug and Play Anisotropy-Based Nanothermometers
S. Thompson, I. Martínez, P. Haro-González, A.P. Adam, D. Jaque, J. Nieder, R.de la Rica, ACS Photonics. (2018)
“…The device control and data analysis were performed with the ISS Vinci software. The temperature of the sample solutions was either controlled or temperature measured at the bottom of the cuvette with Peltier-based temperature controller (Turret 4, Quantum Northwest, Inc.). All the experiments were performed in the same aqueous buffered solution (phosphate buffer saline, PBS) in order to avoid changes in fluorescence polarization anisotropy originating from variations in the viscosity of the solution…”
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Immobilization of a full photosystem in the large pore MIL-101 Metal-organic Framework for CO2 reduction
X. Wang, F. Wisser, J. Canivet, M. Fontecave, and C. Mellot-Draznieks, ChemSusChem. (2018)
“…band pass filter. Samples were prepared in a 1 cm path length quartz cuvette (Starna) which was placed in a temperature controlled cuvette holder (Quantum Northwest) maintained at 20°C with a circulated water bath…”
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Analysis of hydrogels for immobilisation of hepatocytes (HepG2) in 3D cell culturing systems
E. Westergren, DIVA. (2018)
“…Before the Circular Dichroism (CD) measurements all samples were prepared to a (peptide) concentration of 10 µM in PBS. The CD spectra were recorded using the Chirascan Spectropolarimeter from Applied Photophysics with a TC 125 temperature controller from Quantum Northwest steps with the sample in a 1.0 mm quartz cuvette. In the CD two different types of measurements were made; a scan over a wavelength span (200-250 nm, with a step length of 0.5 nm and three repeats per measurement) with fix temperature of 20 ᵒC to detect secondary structure of peptides and a temperature scan over the span 5-90 ᵒC, increasing 1 ᵒC per measurement and 3 repeats, and with a fix wavelength of 222 nm to find the “melting point” of the sample…”
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Design, synthesis and vesicular self-assembly of cyclic, acyclic and lipid based molcules
A. Sapala, Thesis. (2018)
“…For Fluorescence measurements, All the steady-state fluorescence experiments of sample solutions were carried out on the FLS900 spectrofluorometer (Edinburgh, UK). The fluorescence measurements were carried out by using 1 cm quartz cuvettes and the temperature was maintained at 25 ºC using a Peltier based cooler (Quantum Northwest)…”
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Radioprotective role of cyanobacterial phycobilisomes
K. Klementiev, E. Maksimov, D. Gvozdev, G. Tsoraev, F. Protopopov, I. Elanskaya, S. Abramov, M. Dyakov, V. Ilyin, N. Nikolaeva, Y. Slonimskiy, N. Sluchanko, V. Lebedev, A. Spassky, T. Friedrich, G. Maksimov, V. Paschenko, and A. Rubin, bioRxiv (2018)
“…picosecond excitation pulses, driven at a repetition rates up to 50 MHz. Temperature of the sample was stabilized by a Peltier-controlled cuvette holder Qpod 2e (Quantum Northwest, Liberty Lake, not peer-reviewed) is the author/funder. All rights reserved…”
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Production of recombinant human tektin 1, 2, and 4 and in vitro assembly of human tektin 1
M. Budamagunta, F. Guo, N. Sun, B. Shibata, P. FitzGerald, J. Voss, and J. Hess, Cytoskeleton (Hoboken). 75, 3-11 (2018)
“…Circular dichroism spectroscopy (CD) measurements were performed on a Jasco J-715 spectropolarimeter equipped with a Peltier temperature control (Quantum Northwest) set to 25°C. For spectral acquisition, a tektin protofilament sample of 0.1 mg/mL in 5 mM Tris, pH 7.5…”
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Comparison of lipid-dependent bilayer insertion of pHLIP and its P20G variant
V. Vasquez-Montes, J. Gerhart, K. King, D. Thévenin, and A. Ladokhin, Biochim. Biophys. Acta. 1860, 534-543 (2018)
“…emission monochromators. The measurements were made in a 2 × 10 mm cuvette oriented perpendicular to the excitation beam and maintained at 25 °C using a Peltier device from Quantum Northwest (Spokane, WA). For tryptophan…”
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Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP
Y. Slonimskiy, E. Maksimov, E. Lukashev, M. Moldenhauer, C. Jeffries, D. Svergun, T. Friedrich, and N. Sluchanko, Biochim. Biophys. Acta. 1859, 382-393 (2018)
“…recorded as described in [10]. PBs fluorescence quenching was measured as described in [21]. Upon absorption and fluorescence measurements, a blue light-emitting diode (LED) (M455L3, Thorlabs, USA), with a maximum emission at 455 nm was used for the photoconversion of the samples (actinic light (AL) for OCPO→OCPR photoconversion). Temperature of the sample was stabilized by a Peltier-controlled cuvette holder Qpod 2e (Quantum Northwest, USA) with a magnetic stirrer…”
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Towards optimized naphthalocyanines as sonochromes for photoacoustic imaging in vivo
M. Duffy, O. Planas, A. Faust, T. Vogl, S. Hermann, M. Schäfers, S. Nonell, and C. Strassert, Photoacoustics. 9, 49-61 (2018)
“…Samples were measured using a piezoelectric detector 9310 (Quantum Northwest, WA, USA) attached to a fluorescence cuvette in right-angle geometry. The analog signal was amplified using a Panametrics 5662 Preamp (Olympus, Japan) and passed to a Lecroy WaveSurfer 454 oscilloscope (LeCroy, NY, USA) which performed digitalization and signal averaging over 100 shots per measurement. The digital information was transmitted to a PC…”
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Opto-Electrical Properties of Marine Derived Porphyra-334 Mycosporine-Like Amino Acid
Y. Ahn, P. Chopade, J. Lee, H. Kim, H. Seo, V. Kanade, S. Ansari, R. Tongaonkar, S. Park, A Kulkarni, and S. Moh, Journal of Nanoelectronics and Optoelectronics. 13, 415-420 (2018)
“…Porphyra-334 samples were analyzed quantitatively by HPLC (Shimadzu-LC20A, Seattle, WA, USA) with a DAD-SPD M20A detector (Quantum Northwest, Seat- tle, WA, USA) and for MS/MS analysis on an ESI- MS/MS system (Thermo Fisher Scientific, San Jose, CA, USA…”
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Photoisomerization of Styryl Derivatives of Pyridine N-Oxide
Y. Gubarev, N. Lebedeva, E. Yurina, V. Andreev, and A. V’yugin, Russ. J. Phys. Chem. 92, 804-808 (2018)
“…recorded on an AvaSpec- 2048 spectrometer (Avantes Gmbh, the Netherlands) in quartz cells with path lengths of 10 mm at 25°C. To record changes in the spectra, each cell was placed in a temperature-regulated CUV-QPOD cuvette holder (Quantum Northwest, United States…”
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SIRT1 inhibitory compounds from the roots of Codonopsis pilosula
X. Li, L. Cheng, Y. Yan, B. Liu, and Y. Cheng, J Asian Nat Prod Res. 1-8 (2018)
“…Optical rotations were recorded on a JASCO P-1020 digital polarimeter (JASCO Corporation, Tokyo, Japan). UV spectra were collected on a Shimadzu UV-2401PC spectrometer (Horiba, Kyoto, Japan). CD spectra were measured on an Agilent applied photophysics (Quantum Northwest, Inc., Spokane, USA)…”
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N-containing compounds from Periplaneta americana and their activities against wound healing
Y. Yan, B. Xiang, H. Zhu, J. Qi, B. Hou, F. Geng, and Y. Cheng, J Asian Nat Prod Res. 1-10 (2018)
“…Optical rotations were recorded on a JASCO P-1020 digital polarimeter (JASCO Corporation, Tokyo, Japan). UV spectra were recorded on a Shimadzu UV-2401PC spectrometer (Horiba, Kyoto, Japan). CD spectra were measured on an Agilent applied photophysics (Quantum Northwest, Inc., Spokane, U.S.A.)…”
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Design and Characterization of a Singly Labeled Fluorescent Smart Probe for In Vitro Detection of miR-21
S. Oladepo, Appl Spectrosc. 72, 79-88 (2018)
“…Oladepo 81 Page 4. of SP or SP-target duplexes, using a thermoelectrically cooled sample holder inserted in the fluorescence spectro- photometer and a TC125 Temperature Control unit (Quantum Northwest Inc., USA). For…”
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Refining Protein Penetration into the Lipid Bilayer Using Fluorescence Quenching and Molecular Dynamics Simulations: The Case of Diphtheria Toxin Translocation Domain
A. Kyrychenko, N. Lim, V. Vasquez-Montes, M. Rodnin, J. Freites, L. Nguyen, D. Tobias, D. Mobley, and S. Ladokhin, J. Membr. Biol. (2018)
“…emission monochromators. The measurements were made in a 10 × 10 mm cuvette oriented perpendicular to the excitation beam and maintained at 25 °C using a Peltier device from Quantum Northwest (Spokane, WA). For…”
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Improving the spectral measurement accuracy based on temperature distribution and spectra-temperature relationship
Z. Li, J. Feng, P. Liu, Z. Sun, G. Li, and K. Jia, Infrared Physics & Technology. 90, 87-94 (2018)
“…An AvaLight-HAL-S tungsten-halogen lamp (Avantes, Apeldoorn, The Netherlands) was employed as the light source. The temperature in the experiment was controlled by a QNW qpod 2e sample holder of optical path 10 mm (Quantum Northwest, Inc., Liberty Lake, USA)…”
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Laboratory Evolution of Bacillus circulans Xylanase Inserted into Pyrococcus furiosus Maltodextrin-Binding Protein for Increased Xylanase Activity and Thermal Stability Toward Alkaline pH
V. Shah, T. Charlton, and J. Kim, Appl. Biochem. Biotechnol. 184, 1232-1246 (2018)
“…Enzyme Activity Assay. The initial rate of enzymatic hydrolysis of a substrate, ONPX2, was measured by monitoring changes in absorbance at 400 nm over time using a Varian Cary 50 UV-VIS spectrophotometer fitted with a Quantum Northwest Peltier temperature control unit…”
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Time-resolved photoacoustic calorimetry of organometallic ligand substitution in alkane solvents: Molybdenum-alkane σ bond controls fast ligand addition to molybdenum pentacarbonyl
S. Gittermann, and T. Burkey, Chemical Physics. (2018)
“…The transducer signals are amplified and recorded on a digital oscilloscope and a computer. Photoacoustic data were obtained with in-house software AQPAC6 [3a], and analyzed with Quantum Northwest Sound Analysis [14] Quantum Northwest Inc., Liberty Lake, WA…”
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The effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III)
R. Deller, B. Carter, I. Zampetakis, F. Scarpa, and A. Perriman, Biochem. Biophys. Res. Commun. 495, 1055-1060 (2018)
“…Assessment of protein thermal stability. Thermal stability of native and cationized proteins was performed using a Cary 60 UV-visible spectrometer (Agilent Technologies LDA UK Ltd. UK) equipped with a Quantum Northwest TC1 temperature controller (Quantum Northwest Inc.…”
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Effect of the NBD-group position on interaction of fluorescently-labeled cholesterol analogues with human steroidogenic acute regulatory protein STARD1
K. Tugaeva, Y. Faletrov, E. Allakhverdiev, V. Shkumatov, E. Maksimov, and N. Sluchanko, Biochem. Biophys. Res. Commun. 497, 58-64 (2018)
“…pulses as described in Ref. [35]. During the experiment temperature of the sample was controlled by a cuvette holder Qpod 2e (Quantum Northwest, USA). 2.5. Docking simulations. Docking simulations were performed using…”
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Ethanol- and trifluoroethanol-induced changes in phase states of DPPC membranes. Prodan emission-excitation fluorescence spectroscopy supported by PARAFAC analysis
M. Horochowska, K. Cieślik-Boczula, and M. Rospenk, Spectrochim Acta A Mol Biomol Spectrosc. 192, 16-22 (2018)
“…All spectra were corrected for instrument response. The temperature in a cuvette was kept at a constant level of 20 °C for DPPC/ethanol(or TFE) systems by Quantum Northwest TC 125TM thermostat. 2.3. Parallel Factor (PARAFAC) Analysis…”
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Photophysics of a molecular rotor inside the block co-polymers
S. Ahmed, R. Gautam, P. Sharma, and D. Seth, Journal of Photochemistry and Photobiology A: Chemistry. 351, 170-178 (2018)
“…Peltier-controlled cuvette holders from Quantum Northwest (Model: TLC-50) was used for time-resolved measurement to maintain the temperature at 303.15 K and the variation of temperature from 303.15 K to 333.15 K. 3. Results & discussion. 3.1…”
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Temperature dependence of 185 nm photochemical water treatment – The photolysis of water
L. Furatian, and M. Mohseni, Journal of Photochemistry and Photobiology A: Chemistry. 356, 364-369 (2018)
“…The distance from the lamps to liquid surface were made at least five times the aperture to ensure quasi-collimation and held fixed for all experiments. The 185 nm irradiations used a custom made Peltier temperature controlled cell holder (Quantum Northwest, USA)…”
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Photophysics of Thioflavin T in deep eutectic solvents
R. Gautam, S. Ahmed, and D. Seth, Journal of Luminescence 198, 508-516 (2018)
“…The weight-average life time was calculated from the equation 〈 τ 〉 = ∑ i = 1 N C i τ i . In time resolved emission the temperature was varied from 288 to 333 K by using the peltier control cuvette holder from Quantum Northwest (model TLC-50)…”
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Analytical Comparability Assessments of 5 Recombinant CRM197 Proteins From Different Manufacturers and Expression Systems
J. Hickey, V. Toprani, K. Kaur, R. Mishra, A. Goel, N. Oganesyan, A. Lees, R. Sitrin, S. Joshi, and D. Volkin, J Pharm Sci. (2018)
“…Far-UV circular dichroism (CD) spectroscopy was performed using a Chirascan-plus CD spectrometer (Applied Photophysics Ltd., Leatherhead, UK) equipped with a 6-cuvette position Peltier temperature controller (Quantum Northwest, Liberty Lake, WA) and a high…”
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Peptide-Based Fluorescent Probes for Deacetylase and Decrotonylase Activity: Toward a General Platform for Real-Time Detection of Lysine Deacylation
D. Rooker, Y. Klyubka, R. Gautam, E. Tomat, and D. Buccella, Chembiochem. 19, 496-504 (2018)
“…. All spectroscopic experiments were conducted at (37.0:0.1)8C, which was maintained with a Quantum Northwest cuvette temperature controller. HPLC chromatograms were acquired by using the instrumentation described above. Further details about the preparation of solutions of the probes for enzymatic assays, summary of optical properties of the peptides, and calculation of Z’ factors for the assays are described in the Supporting Information…”
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Modulation of Donor-Acceptor Distance in a Series of Carbazole Push-Pull Dyes; A Spectroscopic and Computational Study
J. Sutton, J. Barnsley, J. Mapley, P. Wagner, D. Officer, and K. Gordon, Molecules. 23, (2018)
“…For the 1A–3A emission spectra were collected on the same setup as discussed for resonance Raman, using the Innova I-302 krypton ion laser at 350.7 nm for excitation. While for the rest a diode laser, [448.0 nm (CrystaLaser)] for excitation and a SP2150 (Acton, Princeton Instruments) spectrometer with an air cooled CCD (Pixis 100, Princeton Instruments, Trenton, NJ. USA)) on a 90° bench top setup. A Quantum NorthWest TC125 temperature controller (Liberty Lake, WA, USA) was used to vary temperature for the ranges of 248 K to 293 K across all solvents. Sample concentration was typically 1 × 10−5 mol L−1…”
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The characterization of iron (III) in seawater and related toxicity to early life stages of scleractinian corals
J. Leigh-Smith, A. Reichelt-Brushett, and A. Rose, Environ. Toxicol. Chem. 37, 1104-1114 (2018)
“…Analyses of the 1, 10, and 100 mg/L Fe(III) solutions were conducted using a Cary 50 ultraviolet‐visible spectrophotometer with a 1‐cm path length temperature‐ controlled cuvette holder (Quantum Northwest qpod® 2e‐QNW)…”
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Temperature dependence of water-water and ion-water correlations in bulk water and electrolyte solutions probed by femtosecond elastic second harmonic scattering
Y. Chen, N. Dupertuis, H. Okur, and S. Roke, The Journal of Chemical Physics. 148, 111835 (2018)
“…The sample cell was placed in a customized temperature controller (Quantum Northwest) that provided a precise control of the temperature of the sample. The temperature can be tuned in the range of ☞253.15 K (☞20 ◦C)–423.15 K (150 ◦C) with a precision of ±0.1 K…”
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Order, Disorder, and Temperature-Driven Compaction in a Designed Elastin Protein
K. Greenland, M. Carvajal, J. Preston, S. Ekblad, W. Dean, J. Chiang, R. Koder, and R. Wittebort, J Phys Chem B. 122, 2725-2736 (2018)
“…Coacervation studies were performed using an HP 8452A diode array spectrophotometer ning OLIS SpectralWorks software version 4.9.6 connected to a Quantum Northwest TC125 temperature controller. Solutions were placed in a quartz cuvette and were equilibrated at initial temperature for at least five minutes…”
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A Computational Study of the Ionic Liquid-Induced Destabilization of the Miniprotein Trp-Cage
B. Uralcan, S. Kim, C. Markwalter, R. Prud’homme, and P. Debenedetti, J Phys Chem B. (2018)
“…Temperature ramping at 0.5K/min was carried out by a TC125 temperature controller (Quantum Northwest, Liberty Lake, WA) and a Julabo AWC100 recirculating cooler. Each wavelength was sampled for 25 seconds. Molar ellipticity values were determined from the raw CD data and the known TC5b molarity…”
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One-pot synthesis of dopamine-conjugated hyaluronic acid/polydopamine nanocomplexes to control protein drug release
D. Lim, R. Prim, E. Kang, and S. Jeong, Int J Pharm. 542, 288-296 (2018)
“…The secondary structure of lysozyme was determined by CD spectroscopy, a Chirascan instrument (Applied Photophysics, UK), equipped with a TC125 temperature controller (Quantum Northwest, USA) set at 25 °C. The samples were diluted to desired concentration, loaded…”
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Effects of annealing on the physical properties of therapeutic proteins during freeze drying process
J. Lim, D. Lim, K. Kim, S. Park, and S. Jeong, Int. J. Biol. Macromol. 107, 730-740 (2018)
“…quartz cuvette with light path lengths of 0.2 mm (Hellma Analytics, Mullheim, Germany) was adopted, and the scanning of CD spectra was done at 10 °C. A peltier type temperature controller TC125 was used for controlling temperature (Quantum Northwest Inc., Spokane, USA)…”
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Preferential exclusion mechanism by carbohydrates on protein stabilization using thermodynamic evaluation
N. Kim, R. Thapa, and S. Jeong, Int. J. Biol. Macromol. 109, 311-322 (2018)
“…2.4. Far-UV circular dichroism (CD). CD measurements were performed using the Chirascan-plus spectrometer (Applied Photophysics, Surrey, UK), equipped with a TC125 temperature controller (Quantum Northwest, Spokane, USA) and a temperature probe…”
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NMR based structure reveals groove binding of mitoxantrone to two sites of [d-(TTAGGGT)]4 having human telomeric DNA sequence leading to thermal stabilization of G-quadruplex
S. Tripathi, and R. Barthwal, Int. J. Biol. Macromol. 111, 326-341 (2018)
“…Thermal denaturation data was acquired at 263 nm by using Peltier temperature controller system (Quantum Northwest Peltier) accessory to an accuracy of 0.01 °C. The uncomplexed [d-(TTAGGGT)] 4 and its complex with MTX at D/N = 1.0-3.0 were heated in the temperature…”
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Lanthanum chloride-induced conformational changes of bovine liver catalase: A computational and biophysical study
S. Sharma, R. Samal, U. Subudhi, and G. Chainy, Int. J. Biol. Macromol. 115, 853-860 (2018)
“…in a stoichiometric ratio upto 1:1000. The temperature of cell was adjusted with industrial chiller (cw-3000) and monitored through a temperature control unit (Quantum Northwest TC-425). Each smooth CD spectrum is an average…”
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Light-harvesting complexes of Botryococcus braunii
T. van den Berg, B. van Oort, and R. Croce, Photosyn. Res. 135, 191-201 (2018)
“…for emission. CD spectra were recorded at 20 °C with a Chirascan CD spectrophotometer (Applied Photophysics) equipped with a temperature control unit TC125 (Quantum Northwest). The spectral bandwidth was 1 nm. For…”